WH-Protein A, 100mL Eastbay Online

WH-Protein A is a new type of affinity chromatography resin made by coupling alkali-resistant Protein A with a highly rigid agarosesubstrate by epoxy activation. Compared with traditional antibody affinity media, it has a more stable ligand, faster flow rate, and lower backpressure, and is suitable for the capture of monoclonal antibodies or Fc fusion proteins from high-volume cell cultures, and the capture of polyclonal antibodies from peritoneal fluid or blood plasma. It is also suitable for capturing polyclonal antibodies from ascites or plasma.

The ligand of WH-Protein A is a recombinant protein A fragment obtained from E. coli fermentation. In the upstream construction, base-resistant amino acids are used to replace non-base-resistant amino acids, and protease-sensitive amino acids are removed, which makes this ligand with good stability. The fermentation and subsequent purification processes of the ligand are free of any animal source raw materials. The medium can be cleaned in-situ with 0.1-0.5M NaOH, avoiding the use of expensive and corrosive cleaning reagents, which can effectively save the cost and is easy to be used in industrialized large-scale production.